Reverse transcriptase is an enzyme used by all retrovirus and retrotransposons that transcription the genetic information from the Virus_(biology) or retrotransposon from RNA into DNA, which can integrate into the Host (biology) genome. Eukaryotes with linear DNA uses a variant of reverse transcriptase, called telomerase, with the RNA template contained in the enzyme itself. The enzyme collectively referred to as reverse transcriptase generally includes an RNA-dependent DNA polymerase and a DNA-dependent DNA polymerase, which work together to perform Transcription_(genetics) in the reverse of the standard direction. Usually, transcription only runs from DNA to RNA, catalysis by RNA polymerase. In addition to the transcription function, retroviral reverse transcriptase carries a RNase domain, which belongs to the RNase H family. An example of a reverse transcriptase is the reverse transcriptase from the HIV type 1 (Protein data bank [http://www.rcsb.org/pdb/cgi/explore.cgi?pid=295451034363473&page=20&pdbId=1HMV 1HMV], EC 2.7.7.49). Reverse transcriptase is commonly used in the field of research to be able to apply the polymerase chain reaction technique to RNA. The classical Polymerase chain reaction technique can only be applied to DNA strands, but with the help of reverse transcriptase, RNA can be transcribed into DNA making Polymerase chain reaction analysis of RNA molecules possible. The technique is collectively called: Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Reverse transcriptase is also used to create cDNA library from mRNA. Since HIV use reverse transcriptase, together with integrase, to infect human DNA with viral DNA, reverse transcriptase inhibitors are used to prevent this. The Hepatitis B virus also uses reverse transcriptase, but in a much different way. Reverse transcriptase was discovered by Howard Temin at the University of Wisconsin in the 1970's. David Baltimore independently discovered reverse transcriptase at about the same time, and the two shared the Nobel Prize in medicine for this discovery. ==External links== *[http://nist.rcsb.org/pdb/molecules/pdb33_1.html Reverse transcriptase: PDB molecule of the month] EC 2.7.7 Molecular biology Virology Enzymes Viral enzymes
Are there any other organisms that have RT ? :''I don't think so. What about retrotransposons?''--user:Magnus Manske
See other meanings of words starting from letter:
Words begining with Reverse_transcriptase:
Reverse_transcriptase
Reverse_transcriptase
Reverse_transcriptase_inhibitor
Reverse transcriptase
Reverse transcriptase is an enzyme used by all retrovirus and retrotransposons that transcription the genetic information from the Virus_(biology) or retrotransposon from RNA into DNA, which can integrate into the Host (biology) genome. Eukaryotes with linear DNA uses a variant of reverse transcriptase, called telomerase, with the RNA template contained in the enzyme itself. The enzyme collectively referred to as reverse transcriptase generally includes an RNA-dependent DNA polymerase and a DNA-dependent DNA polymerase, which work together to perform Transcription_(genetics) in the reverse of the standard direction. Usually, transcription only runs from DNA to RNA, catalysis by RNA polymerase. In addition to the transcription function, retroviral reverse transcriptase carries a RNase domain, which belongs to the RNase H family. An example of a reverse transcriptase is the reverse transcriptase from the HIV type 1 (Protein data bank [http://www.rcsb.org/pdb/cgi/explore.cgi?pid=295451034363473&page=20&pdbId=1HMV 1HMV], EC 2.7.7.49). Reverse transcriptase is commonly used in the field of research to be able to apply the polymerase chain reaction technique to RNA. The classical Polymerase chain reaction technique can only be applied to DNA strands, but with the help of reverse transcriptase, RNA can be transcribed into DNA making Polymerase chain reaction analysis of RNA molecules possible. The technique is collectively called: Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Reverse transcriptase is also used to create cDNA library from mRNA. Since HIV use reverse transcriptase, together with integrase, to infect human DNA with viral DNA, reverse transcriptase inhibitors are used to prevent this. The Hepatitis B virus also uses reverse transcriptase, but in a much different way. Reverse transcriptase was discovered by Howard Temin at the University of Wisconsin in the 1970's. David Baltimore independently discovered reverse transcriptase at about the same time, and the two shared the Nobel Prize in medicine for this discovery. ==External links== *[http://nist.rcsb.org/pdb/molecules/pdb33_1.html Reverse transcriptase: PDB molecule of the month] EC 2.7.7 Molecular biology Virology Enzymes Viral enzymes
Reverse transcriptase
Are there any other organisms that have RT ? :''I don't think so. What about retrotransposons?''--user:Magnus Manske
See other meanings of words starting from letter:
R
RA | RB | RC | RD | RE | RF | RG | RH | RI | RJ | RK | RL | RM | RN | RO | RP | RS | RT | RU | RW | RX | RY | RZ |Words begining with Reverse_transcriptase:
Reverse_transcriptase
Reverse_transcriptase
Reverse_transcriptase_inhibitor
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