[[IMAGE:replica plate.PNG|150px|right|frame|Negative selection through replica plating to genetic screen for Amp- colonies]] In molecular biology and microbiology, replica plating is a technique in which one or more secondary Petri plates containing different solid (agar-based) selective medium (lacking nutrients or containing chemical growth inhibitors such as antibiotics) are inoculated with the same colonies of microorganisms from a primary plate (or master dish), reproducing the original spatial pattern of colonies. The technique involves pressing a velvet-covered disk to a primary plate, and then imprinting secondary plates with cells in colonies removed by the velvet from the original plate. Generally, large numbers of colonies (roughly 30-300) are replica plated due to the difficulty in streak (biology) each out individually onto a separate plate. The purpose of replica plating is to be able to compare the master plate and any secondary plates to genetic screen for a genetic marker phenotype. For example, a colony which appeared on the master plate but failed to appear at the same location on a secondary plate shows that the colony was sensitive to a substance on that particular secondary plate. Common screenable phenotypes include auxotrophy and antibiotic resistance Replica plating is especially useful for negative selection. For example, if one wanted to select colonies that were sensitive to ampicillin, the primary plate could be replica plated on a secondary Amp+ agar plate . The sensitive colonies on the secondary plate would die but the colonies could still be deduced from the primary plate since the two have the same spatial patterns from ampicillin resistant colonies. The sensitive colonies could then be picked off from the primary plate. By increasing the variety of secondary plates with different selective medium, it is possible to rapidly screen a large number of individual isolated colonies for as many phenotypes as there are secondary plates. This technique was first described in the cotext of the Luria-Delbruck experiment in 1943. molecular biology microbiology
See other meanings of words starting from letter:
Words begining with Replica_plating:
Replica_plating
Replica plating
[[IMAGE:replica plate.PNG|150px|right|frame|Negative selection through replica plating to genetic screen for Amp- colonies]] In molecular biology and microbiology, replica plating is a technique in which one or more secondary Petri plates containing different solid (agar-based) selective medium (lacking nutrients or containing chemical growth inhibitors such as antibiotics) are inoculated with the same colonies of microorganisms from a primary plate (or master dish), reproducing the original spatial pattern of colonies. The technique involves pressing a velvet-covered disk to a primary plate, and then imprinting secondary plates with cells in colonies removed by the velvet from the original plate. Generally, large numbers of colonies (roughly 30-300) are replica plated due to the difficulty in streak (biology) each out individually onto a separate plate. The purpose of replica plating is to be able to compare the master plate and any secondary plates to genetic screen for a genetic marker phenotype. For example, a colony which appeared on the master plate but failed to appear at the same location on a secondary plate shows that the colony was sensitive to a substance on that particular secondary plate. Common screenable phenotypes include auxotrophy and antibiotic resistance Replica plating is especially useful for negative selection. For example, if one wanted to select colonies that were sensitive to ampicillin, the primary plate could be replica plated on a secondary Amp+ agar plate . The sensitive colonies on the secondary plate would die but the colonies could still be deduced from the primary plate since the two have the same spatial patterns from ampicillin resistant colonies. The sensitive colonies could then be picked off from the primary plate. By increasing the variety of secondary plates with different selective medium, it is possible to rapidly screen a large number of individual isolated colonies for as many phenotypes as there are secondary plates. This technique was first described in the cotext of the Luria-Delbruck experiment in 1943. molecular biology microbiology
See other meanings of words starting from letter:
R
RA | RB | RC | RD | RE | RF | RG | RH | RI | RJ | RK | RL | RM | RN | RO | RP | RS | RT | RU | RW | RX | RY | RZ |Words begining with Replica_plating:
Replica_plating
These materials are based on Wikipedia and licensed under the GNU FDL
YouTube.com videos better site than Turbo Tax 2007
